The Effect of Tadalafil on Anastomotic Healing in Rats, Which Have a Small Intestinal Ischemia Reperfusion
Published: 2020-09-16
Page: 173-181
Issue: 2020 - Volume 3 [Issue 2]
Lütfi Mutlu
Department of General Surgery, Tepecik Training and Research Hospital, Health Sciences University, Izmir, Turkey.
Ramazan Serdar Arslan *
Department of General Surgery, Usak Training and Research Hospital, Usak, Turkey.
Semra Tutcu Şahin
Department of General Surgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
Teoman Coşkun
Department of General Surgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
Eray Kara
Department of General Surgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
Yavuz Kaya
Department of General Surgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Background/Aim: To investigate the effect of specific phosphodiesterase 5 inhibitor on anastomotic healing in rat small intestine which was an ischemia reperfusion injury.
Materials and Methods: 48 male Sprague-Dawley rats divided into four equal groups (n = 12). Standardized transection, a full cut was made approximately 15 centimeters proximal to the cecum and anastomosis were performed to the tips of bowel. Group 1, normal anastomosis; group 2, normal anastomosis + tadalafil treatment; group 3, intestinal anastomosis was performed after 30 minutes of intestinal ischemia and reperfusion; group 4, intestinal anastomosis was performed after 30 minutes of intestinal ischemia and reperfusion + tadalafil treatment. Postoperative 4th day anastomosis bursting pressure, tissue hydroxyproline level, and histopathologically anastamosis evaluation were assessed by modified Ehrlich-Hunt scoring.
Results: The use of Tadalafil in ischemia and reperfusion groups was found to significantly increase the burst pressures. There was a statistically significant difference in hydroxyproline concentration between all groups. There was no statistically significant difference between the groups in histological evaluation in terms of inflammatory cell infiltration, vascular reaction and anastomotic collagen deposition.
Conclusion: Although tadalafil cannot be shown histopathologically, it was concluded that it has positive effects on recovery of small intestine anastomosis after reperfusion of intestinal ischemia.
Keywords: Tadalafil, ischemia, anastomosis, reperfusion, hydroxyproline, burst pressure.