The Effect of Tadalafil on Anastomotic Healing in Rats, Which Have a Small Intestinal Ischemia Reperfusion

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Lütfi Mutlu
Ramazan Serdar Arslan
Semra Tutcu Şahin
Teoman Coşkun
Eray Kara
Yavuz Kaya


Background/Aim: To investigate the effect of specific phosphodiesterase 5 inhibitor on anastomotic healing in rat small intestine which was an ischemia reperfusion injury.

Materials and Methods: 48 male Sprague-Dawley rats divided into four equal groups (n = 12). Standardized transection, a full cut was made approximately 15 centimeters proximal to the cecum and anastomosis were performed to the tips of bowel.  Group 1, normal anastomosis; group 2, normal anastomosis + tadalafil treatment; group 3, intestinal anastomosis was performed after 30 minutes of intestinal ischemia and reperfusion; group 4, intestinal anastomosis was performed after 30 minutes of intestinal ischemia and reperfusion + tadalafil treatment. Postoperative 4th day anastomosis bursting pressure, tissue hydroxyproline level, and histopathologically anastamosis evaluation were assessed by modified Ehrlich-Hunt scoring.

Results: The use of Tadalafil in ischemia and reperfusion groups was found to significantly increase the burst pressures. There was a statistically significant difference in hydroxyproline concentration between all groups. There was no statistically significant difference between the groups in histological evaluation in terms of inflammatory cell infiltration, vascular reaction and anastomotic collagen deposition.

Conclusion: Although tadalafil cannot be shown histopathologically, it was concluded that it has positive effects on recovery of small intestine anastomosis after reperfusion of intestinal ischemia.

Tadalafil, ischemia, anastomosis, reperfusion, hydroxyproline, burst pressure.

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Mutlu, L., Arslan, R. S., Şahin, S. T., Coşkun, T., Kara, E., & Kaya, Y. (2020). The Effect of Tadalafil on Anastomotic Healing in Rats, Which Have a Small Intestinal Ischemia Reperfusion. Asian Journal of Research in Surgery, 4(2), 21-29. Retrieved from
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